High-throughput nanowell-based image-verified cloning technology (HT-NIC) is a new method allowing the fast generation of clonal pharmaceutical production cell lines. Clones are generated in just one cloning round while providing robust in-process image-verified monoclonality proof.
This application guide from Sartorius presents a workflow for CellCelector HT-NIC technology, highlighting the integrated monoclonality and viability assessment of clones, as well as outgrowth rates after clone transfer into 96- or 384-well plates.
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