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Peptide Barcodes for Next-Generation Protein Sequencing

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"Peptide Barcodes for Next-Generation Protein Sequencing"

Barcoding technologies that take advantage of the high information content that can be encoded in oligonucleotide sequences have enabled an array of applications in biotechnology, particularly when coupled with next-generation DNA sequencing (NGS) to decode this information in a high-throughput, cost-effective manner.

For example, DNA barcoding combined with NGS readout is employed to track sample identity in multiplexed libraries, provide single-cell or spatial resolution in transcriptomic studies, and track the enrichment of genotypes in directed evolution methods for protein engineering. Peptide barcodes, with their easy genetic encoding, chemical versatility, and capacity to encode extensive information in short sequences, present novel opportunities.

This application note from Quantum-Si presents a novel protein barcoding approach using next-generation protein sequencing, providing criteria for peptide barcode design, methods for generating peptide barcode libraries, and example applications that illustrate how peptide barcodes can be used for screening proteins with desired properties.

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