FFPE tissue and samples with a low abundance of high-quality nucleic acid represent a bottleneck in sample preparation workflows.
Researchers and clinicians working with these samples often resort to labor-intensive and potentially biased methods in order to isolate sufficient material for downstream analyses.
Purigen’s Ionic Purification System uses isotachophoresis to separate and concentrate DNA and RNA in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Ionic Fluidic Chip. An electric field is then applied to the chip and the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there’s no binding to, or stripping from, fixed surfaces. The result is a higher yield of pure nucleic acid that is less fragmented and free from bead or wash buffer contamination. The new Ionic FFPE Complete Purification Kit enables extraction of both DNA and RNA (including miRNA) simultaneously from FFPE samples in a single workflow.
In this webinar, we share results and data (from Purigen and Canopy Biosciences) from downstream molecular analyses of DNA and RNA that was performed with the new Ionic FFPE Complete Purification Kit.
In the webinar you will learn:
• Purigen Biosystems’ innovative approach to automated nucleic acid purfication using isotachophoresis
• How the Ionic Purification System can be used to extract more DNA and RNA from challenging FFPE samples with more reliability and less effort by comparison to conventional extraction methods
Offered Free by: Purigen Biosystems
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