The use of microscopy for identification of specific chromosomes can be a challenge when working with organisms for which only limited cytogenetic tools, if any, exist. Target-specific DNA Fluorescent in situ Hybridization (DNA FISH) is a powerful technique for the detection of specific chromosomal loci.
The myTags product line from Arbor Biosciences provides services for the design and manufacturing of synthetic FISH probes that delivers computer-designed custom probe sets specific to their target sequences. With these techniques, it is possible to produce a highly specific collection of probes targeting one or more loci on each chromosome. By carefully selecting the number of loci to detect per chromosome, identifying their locations on each arm, and using more than one labeling color, it is possible to uniquely encode every single chromosome of a given species. Each condensed chromosome on a metaphase spread can be clearly identified by its unique pattern of colored spots, in a technique known as “chromosome indexing”.
In this white paper, we present several published applications of chromosome indexing in plants, and outline major points of consideration when undertaking new chromosome indexing projects for different species. This easy-to-use technique can be applied to address many critical genomic research questions in plants, animals, insects, or any other species.
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