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Genomic DNA anomalies are important biomarkers and drug targets in many cancer types.

DNA in-situ hybridization (ISH) is the standard method to directly visualize these molecular alterations, but currently available fluorescent ISH (FISH) assays provide limited morphological detail due to the use of fluorescent nuclear staining as compared to chromogenic staining. Furthermore, FISH techniques rely on fluorescence microscopes, risk the loss of fluorescent signal over time, and involve imaging at high magnifications. There is thus an unmet need for a sensitive and robust chromogenic DNA ISH assay that can enable high-resolution detection of genomic DNA targets with the ease of bright-field microscopy.

This poster from ACD, presented at AACR 2021, demonstrates use of a novel chromogenic ISH assay to detect DNA copy number and structural variations, showing specific and efficient detection of gene rearrangements (ALK), amplifications (ERBB2, EGFR, MET), and deletions (TP53 and CDKN2A) without specialized equipment, providing an alternative to commonly used FISH assays in many cancer research applications.

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