The nervous system consists of numerous specialized cell types that remain to be fully characterized at the molecular level.
Due to the high degree of structural and functional heterogeneity and the intricate spatial organization of these cells, it is of special importance to analyze gene expression within the morphological and spatial tissue context.
The RNAscope in situ hybridization (ISH) assay enables highly sensitive and specific gene expression visualization within the nervous system. BaseScope is an ISH assay that allows visualization of splice junctions between adjacent exons and/or retained introns in order to characterize alternative splicing events in cells and tissues.
This application note highlights case studies for both ISH assays in the nervous system.
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