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"Use of Quantitative Mycoplasma DNA Controls in Evaluating the Sensitivity of Molecular-Based Detection Systems"

Mycoplasma contamination affects approximately 15-35 percent of continuous cell cultures, causing various detrimental effects such as chromosomal abnormalities and inhibition of cell metabolism.

Traditional culture-based techniques and DNA staining with fluorochromes have been used for mycoplasma testing in research laboratories; however, these methods are laborious, costly, and have undefined limits of detection. As a result, there is a need for alternative molecular-based detection systems with improved sensitivity and efficiency.

ATCC has designed quantitative mycoplasma DNA controls for use in evaluating the sensitivity of molecular-based detection systems. This white paper from ATCC demonstrates the use of these controls in assessing two different molecular-based detection systems: Bio-Rad QX100 Droplet Digital PCR and CFX96 Touch Real-Time PCR platforms. Both systems were found to sensitively detect and quantify mycoplasma DNA, with the CFX96 Touch platform exhibiting higher accuracy at lower concentrations of genomic material.

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