Research Library

The top resource for free research, white papers, reports, case studies, magazines, and eBooks.

Share Your Content with Us
on TradePub.com for readers like you. LEARN MORE
UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel Carbon LC Column
Request Your Free White Paper Now:

"UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel Carbon LC Column"

Analysis of vitamin D metabolites has continued to be a topic of interest in recent publications, primarily as biomarkers for possible disease states and vitamin deficiency.

While vitamin D is present in two forms, vitamin D2 and vitamin D3, current ELISA methods demonstrate different cross-reactivities and cannot distinguish between D2 and D3 forms of the vitamin metabolites, resulting in erroneous reporting of total 25-hydroxyvitamin D concentrations. Further, there is interest in an analytical means to differentiate the D2 and D3 forms from the D2 and D3 epimers because of their different degrees of bioactivity.

This application note from Millipore Sigma demonstrates the use of the Supel Carbon LC ultra-high-performance liquid chromatography column to resolve vitamin D2 and D3 metabolites and their epimers with high sensitivity.


Offered Free by: Millipore Sigma
See All Resources from: Millipore Sigma

Recommended for Professionals Like You:

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.