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The Use of Molecular Barcodes in Anchored Multiplex PCR Chemistry

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"The Use of Molecular Barcodes in Anchored Multiplex PCR Chemistry"

This white paper from IDT describes how to use anchored multiplex PCR chemistry, along with the Archer Analysis informatics platform, for sample tagging and multiplexing, as well as the advantages of molecular barcodes in targeted sequencing and variant calling.

Sample barcoding, also called sample indexing, is a common approach to labeling samples for multiplex sequencing and analysis. All nucleic acids in a sample are labeled with the same sequence tag, and the resulting library is pooled with other libraries and sequenced in parallel in a single run. Then, during analysis, the sample-specific indexes enable the software to separate the multiplexed sequence data into sample-specific data sets.

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