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Targeted Sequencing of SARS-COV-2: Swift RNALibrary Kit and Arbor Biosciences myBAITS Expert Virus Panel
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"Targeted Sequencing of SARS-COV-2: Swift RNALibrary Kit and Arbor Biosciences myBAITS Expert Virus Panel"

The emergence of SARS-CoV-2 has resulted in the need for cost-effective and rapid strategies to characterize the viral genome. However, maximizing viral RNA sequencing reads from clinical specimens or laboratory samples can be challenging, particularly due to the amount of host background that may be present.

Here, we present a targeted next-generation sequencing strategy that combines the Swift RNA Library Kit with the Arbor Biosciences myBaits Expert Virus SARS-CoV-2 panel. This approach has the following benefits: 

  • Detection down to 10 viral genome copies in a background of 50 ng human gRNA
  • >99.9% coverage of the SARS-CoV-2 full-length genome
  • Enrichment rates over 100,000-fold
  • >100X genome coverage depth with fewer than 100,000 paired-end reads
  • Multiplex up to 384 samples in a single MiSeq run with >50X coverage per sample


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