Research Library

The top resource for free research, white papers, reports, case studies, magazines, and eBooks.

Share Your Content with Us
on TradePub.com for readers like you. LEARN MORE
Targeted Pathogen Genomics via NGS Hybridization Capture
Request Your Free White Paper Now:

"Targeted Pathogen Genomics via NGS Hybridization Capture"

Next-generation sequencing (NGS) is a powerful method for rapid whole-genome sequencing of microbial genomes or overall microbial species content in complex samples. However, most biological samples with microbes of interest are dominated by non-microbial DNA. This necessitates extremely deep sequencing in order to accurately resolve microbial genomes, or to fully characterize the variation within microbial communities.

Targeted sequencing, wherein background non-target DNA is excluded from samples prior to sequencing, solves this problem and drastically reduces the overall costs of sequencing and data analysis per sample. Target sequence enrichment typically involves either PCR amplification with site-specific primer pairs or hybridization-based capture with target specific biotinylated probes. Given the high degree of sequence variation within and between viral or bacterial strains, proper primer binding for amplicon formation can be inconsistent. Thus hybridization capture is currently the most versatile technique currently for comprehensive, cost-effective sequencing of both viruses and bacteria in complex samples.


Offered Free by: Arbor Biosciences
See All Resources from: Arbor Biosciences

Recommended for Professionals Like You:

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.

Thank you

This download should complete shortly. If the resource doesn't automatically download, please, click here.