This application note from Q2 Lab Solutions explores the capabilities of single-cell RNA sequencing (scRNA-seq) of formalin-fixed paraffin-embedded (FFPE) tissues using the 10x Genomics Chromium Single Cell Gene Expression Flex assay. It highlights the assay's ability to provide high-quality, reproducible gene expression data from FFPE samples, overcoming previous limitations due to RNA fragmentation in fixed tissues.
The note details the experimental procedures and quality control metrics, showing that the assay effectively captures a wide range of gene expressions across different tissue types and sample conditions, with a strong correlation between scRNA-seq and bulk RNA-seq data. The findings support the use of this assay in clinical research for detailed cellular analysis and biomarker discovery in archived tissue samples.
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