3D human in vitro cell models are gaining increased importance in the drug discovery and development cycle of the pharmaceutical industry as they mimic the human in vivo responses of newly developed substances more precisely than 2D models.
They can help to bridge the gap between the traditional 2D in vitro models and whole-animal systems, as the traditional models do not effectively recapitulate the more complex signaling and cellular interactions found in vivo. Spheroids are scaffold-free 3D cell culture models that are aggregated from one or more organ-specific cell types.
InSphero has developed 3D cell-based assay solutions in the fields of liver toxicology, metabolic diseases, and oncology. Industrial use of these complex in vitro systems requires a perfect match of the tissue model, cell culture plates, and liquid handling instrumentation. The scalable format requires liquid handling systems for convenient, yet quick, reliable, and precise medium exchange, sampling, dosing, and spheroid transfer from multiple plates.
This application note from Integra Biosciences describes a semi-automated protocol using an automated pipetting system for cell seeding and medium exchange for assays using spheroids, in vitro scaffold-free 3D cell culture models that are aggregated from one or more organ-specific cell types.
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