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SARS-CoV-2 Amplicon Sequencing
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"SARS-CoV-2 Amplicon Sequencing"

The COVID-19 pandemic precipitated one of the most concentrated scientific efforts ever focused on a single pathogen.

Worldwide, academic, government, and commercial institutions pivoted from their usual endeavors to study the epidemiology, genomics, biochemistry, and evolution of the SARS-CoV-2 virus and develop diagnostic tests, treatments, and vaccines. One unforeseen consequence of these efforts was a global bottleneck in the supply of synthetic DNA from suppliers relying on highly centralized phosphoramidite-based production and third-party logistics.

This application note from DNA Script demonstrates the use of primers produced with their novel enzymatic DNA synthesis (EDS) technology for whole-genome amplicon sequencing and phylogenetic analysis of SARS-CoV-2 isolates using the ARTIC network protocol, showing EDS primers to perform comparably to commercial primers.


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