Research Library

The top resource for free research, white papers, reports, case studies, magazines, and eBooks.

Share Your Content with Us
on TradePub.com for readers like you. LEARN MORE
RNAscope LS Multiplex Fluorescent Assay Combined with Immunofluorescence: Integrated Co-Detection Workflow (ICW) on Leica Bond RX
Request Your Free White Paper Now:

"RNAscope LS Multiplex Fluorescent Assay Combined with Immunofluorescence: Integrated Co-Detection Workflow (ICW) on Leica Bond RX"

This technical note provides guidelines for performing automated fluorescent co-detection of RNA and protein by combining in situ hybridization (ISH) using an RNAscope LS Multiplex Fluorescent Reagent Kit (Cat. No. 322800) and immunofluorescence (IF) on the Leica BOND RX System.

You can use these protocols with a wide range of protein targets, including those that are protease sensitive. On the Leica BOND RX, the samples undergo deparaffinization and heat-induced epitope retrieval, followed by primary antibody incubation and post-primary fixation. Samples are then treated with protease and undergo probe hybridization and full LS Multiplex RNAscope detection, followed by secondary antibody incubation, detection, and counterstaining. 


Offered Free by: ACD
See All Resources from: ACD

Recommended for Professionals Like You: