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"Reverse Transcription Cycling Achieves Greater Than 100 Percent cDNA Conversion with Implications for Ultra-High Sensitivity of Clinically-Actionable, Oncogenic RNA Mutations"
"Reverse Transcription Cycling Achieves Greater Than 100 Percent cDNA Conversion with Implications for Ultra-High Sensitivity of Clinically-Actionable, Oncogenic RNA Mutations"
Oncogenic RNAs are biomarkers with diagnostic, prognostic, and predictive clinical implications in many cancers and specimen types. Methods for detecting oncogenic RNAs typically require reverse transcription to cDNA, which is vulnerable to RNA drop-outs at low copy numbers.
This poster from Asuragen describes the development of a linear amplification method using a thermostable reverse transcriptase, an RNA-protective buffer, and high-temperature cycling, and demonstrates that RNAs are amplified by at least 10-fold through cDNA synthesis, leading to accurate quantification of variants missed using conventional reverse transcription.
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