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"Preclinical CAR-T Cell Target Safety, Biodistribution, and Tumor Infiltration Analysis Using the RNAscope ISH Assay"
"Preclinical CAR-T Cell Target Safety, Biodistribution, and Tumor Infiltration Analysis Using the RNAscope ISH Assay"
In this report we illustrate a way to detect activated CAR-T cells in the tissue context, as well as low expression of CAR target antigen, using the RNAscope in situ hybridization (ISH) technology.
CAR-T cells are activated by target antigen engagement even at very low levels of target expression. In order to avoid adverse events resulting from “on-target/offtumor” toxicity, a highly sensitive and specific gene expression assay is necessary for preclinical target safety assessment.
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