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PCR Product Purification with QiaQuick 96 PCR Purification Kit and the Viaflo 96 Handheld Electronic Pipette
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"PCR Product Purification with QiaQuick 96 PCR Purification Kit and the Viaflo 96 Handheld Electronic Pipette"

The QiaQuick 96 PCR Purification Kit is suitable for purifying up to 10 μg of material for downstream applications, such as sequencing, cloning, labeling, and microarrays. The kit facilitates the removal of impurities like primers, unincorporated nucleotides, buffers, salts, mineral oils, agarose, and enzymes. The vacuum-driven process is faster than centrifugation and gives high, reproducible yields.

Although QiaQuick 96 provides a high-throughput solution, the elution, washing, and binding steps are very laborious and time consuming if performed manually. With the Viaflo 96 handheld electronic pipette, the hands-on time is reduced, as samples and reagents can be transferred to all 96 wells at once. This enables rapid and efficient high-throughput PCR clean-up.

This application note from Integra Biosciences describes how to purify PCR products of impurities with Qiagen’s QiaQuick 96 PCR Purification kit and the Viaflo 96 handheld electronic pipette, which reduces hands-on time improves reproducibility.


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