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Optimizing RNAscope ISH Image Analysis using HALO Image Analysis Software
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"Optimizing RNAscope ISH Image Analysis using HALO Image Analysis Software"

In this 60-minute on-demand webinar, Ghislaine Lioux presents solutions to common RNAscope image analysis challenges, including how to optimize color deconvolution, nuclear segmentation, and how to manage spot clusters.

A recommended end-to-end workflow, from selecting an imaging modality to validating image analysis results will be presented. The webinar presents a live demonstration of HALO image analysis on optimization of in situ hybridization (ISH) analysis, fluorescence in situ hybridization (FISH) analysis, and protein/ nucleic acid co-detection assay.

Learning Objectives:

  • How to address common image analysis challenges in RNAscope assays.
  • How to optimize HALO image analysis settings for ISH, FISH, and codetection assays such as ISH-IHC and FISH-IF.
  • The checkpoints for RNAscope quantitative image analysis success.


Offered Free by: ACD
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