Fragment analysis outputs the size of DNA fragments in a sample using capillary electrophoresis, during which negatively charged DNA molecules are separated by size as they migrate through the capillary toward a detection cell.
Using multiple fluorophores allows fragments of similar sizes to be further distinguished when multiplexing. The various targets are all identified from the same sample in the same reaction, giving fragment analysis an advantage over qPCR. Fragment analysis is also cheaper and faster than NGS and allows for simpler data analysis and interpretation.
This white paper from Thermo Fisher Scientific summarizes a webinar in which the speakers discussed using fragment analysis for the multiplexed identification of viruses and bacteria, focusing on applications for artificial joint infections, inflammatory bowel disease, and improving in vitro fertilization success rates.
The live webinar was hosted by Nature Research Custom Media and sponsored by Thermo Fisher Scientific.
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