RNA sequencing (RNA-seq) has become an essential tool in many research fields, covering a wide range of organisms and systems. However, generating accurate and informative RNA-seq data for transcriptomic study can be difficult due to the exceedingly high proportion of ribosomal RNA (rRNA).
Although several rRNA removal strategies have been developed to aid in RNA-seq library preparation, probe-free rRNA depletion has been reported as more versatile compared to other methods, especially for its potential universal compatibility with different classes of organisms. Zymo-Seq RiboFree Total RNA Library Kit has been developed and optimized based on this novel rRNA depletion strategy, which has been quickly adopted by researchers for transcriptomic studies in animals, plants, and microorganisms.
This application note from Zymo Research describes a whole-transcriptome analysis on several model organisms using the Zymo-Seq RiboFree Total RNA Library Kit to validate its cross-species compatibility and introduces a complete workflow from RNA extraction to bioinformatic analysis.
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