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The MTT cell proliferation assay is a commonly used colorimetric assay for assessing cellular metabolic activity.

This broadly used, rapid, and quantitative cell-based assay can be used to measure cell proliferation, cytotoxicity, or cell activation for a variety of cell types. The test is based on the reduction of yellow tetrazolium salt (MTT) to purple formazan crystals by metabolically active cells; viable cells contain NADH-dependent oxidoreductase enzymes which reduce the MTT to formazan. The insoluble crystals are dissolved using a solubilization solution with the darker the color of the resulting solution, the greater the number of metabolically active cells.

This application note from Integra Biosciences provides a step-by-step protocol for automating the MTT cell proliferation assay using the Assist Plus pipetting robot to improve throughput, reproducibility, safety, and reagent efficiency.

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