The immune response to tumors is a complex, multifactorial interaction that is shaped by the host, the tumor, and the tumor microenvironment (TME).
Interactions between tumor cells and surrounding immune cells in the TME play a key role in tumor progression and treatment response, with accumulating evidence indicating a crucial role for tumor infiltrating immune cells. Immune cells can inhibit tumor growth and progression by recognizing and attacking malignant cells, but immune cells can also promote tumor cell growth, survival, and angiogenesis. Immunotherapies have demonstrated therapeutic efficacy and durable response for several tumor types, however the majority of patients are resistant or relapse after initial response.
More precise characterization of the tumor and its microenvironment with a highly multiplexed, spatial approach can provide critical insight into new immunotherapeutic strategies and identify new predictive biomarkers for stratifying patients most likely to benefit from immunotherapy.
In this white paper we describe a new automated workflow that uses the RNAscope in situ hybridization (ISH) technology to molecularly guide the GeoMx Digital Spatial Profiler (DSP) in detecting highly multiplexed gene expression with spatial resolution.
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