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miRNAscope: A Novel In Situ Hybridization Technology for the Detection of Small Regulatory RNAs with Spatial and Morphological Context
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"miRNAscope: A Novel In Situ Hybridization Technology for the Detection of Small Regulatory RNAs with Spatial and Morphological Context"

microRNAs (miRNAs) are 18 to 22 nucleotide small regulatory RNA molecules that are expressed in a tissue-, cell type-, and cell state-specific manner.

Attempts at cataloging miRNAs have relied on small RNA sequencing approaches, microarray, and quantitative polymerase chain reaction (qPCR). While these methods deliver bulk expression levels, they do not provide detailed spatial information for miRNA expression. 

To address this need, ACD developed an RNA ISH method known as miRNAscope, which offers a “Ready to Use” small RNA detection ISH assay supplied with universal tissue pretreatment reagents and zero target probe optimization.

This poster describes how the miRNAscope assay detects small RNA species with unparalleled detection sensitivity and specificity, enabling morphological information with single-cell resolution.


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