Wastewater testing is an important tool for public health and industrial monitoring programs. However, wastewater samples consist of a complex matrix with high concentrations of organic compounds that are inhibitory to nucleic acid extraction and downstream processing. Hence, it is important to eliminate these contaminants during the extraction process to make samples amenable to downstream applications such as sequencing and quantitative PCR (qPCR).
This application note from Thermo Fisher Scientific describes a study utilizing whole-genome sequencing and 16S rRNA gene sequencing to identify microorganisms present in wastewater samples, demonstrating nucleic acid isolation with the MagMax Wastewater Ultra Nucleic Acid Isolation Kit, which offers multiple workflow options for wastewater and sludge samples.
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