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"Cas9 Plasmids vs Proteins: How Your CRISPR Gene Editing Experiments Can Benefit from Cas9 Proteins"

Traditional approaches to delivering Cas9 and the synthesis of guide RNA (gRNA), such as plasmids present several limitations. The introduction of gRNA ribonucleoprotein (RNP) complexes has helped overcome these challenges, providing an effective delivery method for CRISPR editing tools for a variety of mammalian cell engineering applications.

This infographic from Thermo Fisher Scientific discusses the advantages of using Cas9 proteins over traditional plasmids in CRISPR gene editing, highlighting their efficiency, robustness, and lower risk of off-target effects, particularly for challenging cell lines like induced pluripotent stem cells and primary T cells.

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