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"Achieve High Specificity and On-Target Efficiency with TrueCut HiFi Cas9 Protein"

Off-target effects can have undesirable consequences in therapeutic CRISPR-Cas9 genome editing applications.

In this application note from Thermo Fisher Scientific, the authors detail their efforts to identify a Cas9 variant that would limit off-target edits, using a target-enriched GUIDE-seq (TEG-seq) to screen several Cas9 candidates. They present their development of a high-fidelity Cas9 protein, TrueCut HiFi Cas9, which was optimized for editing efficiency and showed higher fidelity than wild-type TrueCut Cas9 Protein v2 and other commercially available high-fidelity Cas9 proteins in a variety of cell types.

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