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"Immunoprecipitation of IL-6 From Human Serum for Next-Generation Protein Sequencing on Platinum"

The enrichment of low-abundant proteins from complex biological matrices is a key step for many proteomic workflows.

This technical note from Quantum-Si presents an immunoprecipitation protocol compatible with Quantum-Si’s next-generation protein sequencing workflow and utilizes an on-bead digestion protocol that forgoes the sample elution step and proceeds directly to the preparation of digested peptide libraries. The technical note demonstrates the feasibility of this approach by specifically enriching IL-6 through immunoprecipitation from human serum, followed by protein sequencing and identification with the Platinum benchtop protein sequencing instrument.

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