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"Investigation of Microsatellite Instability in RNA Compared to DNA, Using Microsatellite Targeted, Anchored Multiplex PCR and NGS"

The authors of this poster from IDT investigated the status of microsatellites using anchored multiplex (AMP) PCR-targeted panels and compared results from total nucleic acid inputs using an AMP MSI module with VariantPlex chemistry to interrogate DNA and FusionPlex chemistry to interrogate both RNA and DNA.

MSI measurements are typically made on DNA, but it would be useful to make similar measurements from RNA or total nucleic acid inputs so that MSI determinations could be made in parallel to the interrogation of the RNA. Doing so requires characterizations of microsatellite lengths from those that are transcribed and untranscribed because both will be detected following cDNA creation. Microsatellite instability (MSI) occurs when small unit repeats of DNA undergo incorrect replication resulting in the growth or contraction of the number of repeats in the newly synthesized DNA. This accumulation of errors in microsatellite DNA is usually mitigated by the mismatch repair (MMR) pathway which can correct these errors. MSI is of interest because it serves as a phenotypic readout of the status of the MMR machinery, and MSI status can correlate to tumor progression and response to agents that regulate these cellular mechanisms.

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