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"The Callisto Sample Prep System Supports Robust and Versatile Sample Prep for High-Quality HiFi Long-Read Sequencing"

This application note from Volta Labs demonstrates the extraction of high-quality high molecular-weight DNA and the preparation of SMRTbell libraries in different reaction formats, from a range of inputs, and with different workflow parameters, generating high-quality HiFi sequencing data and variant calls with minimal training and a significant reduction in hands-on time.

Highly accurate (HiFi) long-read SMRT (single-molecule real-time) DNA sequencing on the PacBio platform is now commonly used, particularly for applications where short-read NGS has been proven to be inadequate. These include de novo assembly of haplotype-resolved reference genomes, detection and characterization of large structural variants, interrogation of challenging genomic regions, direct methylation analysis, and metagenome deconvolution. Recent advances in the accuracy, throughput, and speed of HiFi sequencing — combined with a significant reduction in per-base cost — promise to broaden access and accelerate the adoption of long-read sequencing in life sciences research and the clinic. Proportionate improvements in the scalability, robustness, and cost of sample preparation for HiFi sequencing are needed.

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