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"Characterization of Germinal Center and Circulating T Follicular Helper Cells Using Both Intracellular and Surface CITE-seq"

This poster from BD Life Sciences describes a study characterizing heterogeneous subsets of TFH and cTFH cells using a combination of intracellular CITE-seq, surface CITE-seq, and whole-transcriptome single-cell RNA sequencing analysis, demonstrating how the combination of data can be used to deepen understanding of the differentiation states and subsets of cells at low abundance.

T follicular helper (TFH) cells are a CD4+ T cell subset that plays a critical role in the formation of germinal centers (GC). TFH cells are essential for producing protective antibody responses through the regulation of clonal selection and differentiation into activated antibody-secreting and memory B cells. TFH cell differentiation is a multi-step process that begins with the presentation of antigen by dendritic cells to naive CD4+ T cells, where the CD4+ T cells commit to the TFH lineage by expressing the lineage-defining transcription factor B cell lymphoma 6 (Bcl-6). TFH cells are phenotypically characterized by the high expression of C-X-C chemokine receptor 5 (CXCR5) and programmed cell death-1 (PD-1) on the cell surface. CXCR5+ memory CD4+ T cells present in peripheral blood represent a circulating TFH (cTFH) cell subset. In contrast to TFH cells, cTFH cells may express low levels of Bcl-6 with numerous reports of subsets that differ both phenotypically and functionally.

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