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"Protein Identification Using Next-Generation Protein Sequencing of In-Gel Digested Proteins"

Western blot is one of the most widely used methods for protein identification but achieving reliable and reproducible results depends on quality antibodies with validated specificity for the target protein. Furthermore, differences in amino acid sequences and isoforms cannot be easily differentiated by western blot if the size of the protein is similar. To overcome these limitations, non-antibody-based approaches are highly desired.

This technical note from Quantum-Si describes an in-gel protein digestion and peptide extraction procedure compatible with Quantum-Si’s library preparation and protein sequencing workflows that can be used to separate and enrich single proteins from complex mixtures, followed by identification on the Platinum protein sequencing instrument.

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