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Quality Assessment of NGS Libraries Using Agilent Automated Electrophoresis Systems

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"Quality Assessment of NGS Libraries Using Agilent Automated Electrophoresis Systems"

Next-generation sequencing (NGS) has become essential to many molecular biology laboratories for studying genetic variation associated with disease or other biological research.

Generation of robust NGS results relies largely on the quality of the library, making analysis of the libraries a crucial step in the sequencing workflow. Thus, many library preparation protocols recommend quality control of the input sample throughout the workflow and of the final library to be sequenced. Knowledge of a library’s size and concentration provides information that is crucial for preparing the sequencer to achieve optimal data output and enhance confidence in sequencing results.

This paper from Agilent provides data highlighting the capability of Agilent automated electrophoresis instruments to accurately size and quantify NGS libraries to aid in generating robust sequencing results.

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