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Exosomal miRNA Analysis with Agilent Bioanalyzer and SurePrint miRNA Microarray
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"Exosomal miRNA Analysis with Agilent Bioanalyzer and SurePrint miRNA Microarray"

Exosomal microRNA (miRNA) is a unique type of RNA that is important in cell-cell communication and is protected from RNase degradation due to being enclosed in the exosome.

Recently, exosomal miRNA has gained interest as a minimally invasive biomarker for liquid biopsies. However, these miRNAs can be difficult to detect and analyze due in part to their low abundance.

This application note describes a study that tested the ability of various kits to extract RNA from 1 mL or 3.7 mL of serum with exosomal enrichment, then performed quality control with the 2100 Bioanalyzer and analyzed miRNAs with the SurePrint G3 Human miRNA microarray.

The study indicates the Agilent miRNA microarray is a powerful tool to detect and analyze miRNAs from liquid biopsy without modifying the standard microarray procedure.


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