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Digital Fluidics Enables Broad-Based Access to Push-Button NGS Sample Prep

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"Digital Fluidics Enables Broad-Based Access to Push-Button NGS Sample Prep"

This poster from Volta Labs describes a benchtop automated sample prep system and presents three case studies demonstrating the system’s ability to produce higher yields of quality DNA, produce libraries from lower inputs, and produce slightly better variant calling than standard manual protocols.

Decades and billions of dollars have been dedicated to advancing sequencing technologies and genomic data analysis. This has resulted in a reduction in sequencing cost by six orders of magnitude and an explosion in sequencing-based applications. However, the sample preparation process remains a significant bottleneck. When samples are processed manually, variable sample quality, laborious protocols, and high per-sample cost remain significant obstacles to scalability and consistency. Automated liquid handlers enable higher throughput, and process but significant barriers to implementation persist. These include high capital expenditure, the need for multiple, dedicated instruments, specialized infrastructure and/or skills, lengthy method development, and ongoing maintenance costs.

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