An important aspect of drug discovery is understanding the interaction of the drug candidate with plasma proteins and lipids to understand the amount of free drug available in blood.
Equilibrium membrane dialysis has been the traditional technique to measure drug-protein binding. This technique involves equilibration of the drug-rich plasma sample with the drug-free buffer across a membrane, allowing for free drug to migrate across the membrane and preventing the transfer of the protein-bound drug into the buffer. This equilibrium is achieved over 24 hours. Other techniques such as rapid equilibrium dialysis further reduce the workflow time down from >24 hours to 6 hours using specifically designed devices.
This white paper from Millipore Sigma demonstrates use of the Supel BioSPME 96-Pin device to measure drug-protein binding to understand the amount of free drug available in blood, finding the technique to save significant time as compared with rapid equilibrium dialysis techniques.
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