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Comparison of Manual and Automated NGS Library Preparation Using the Magnis Dx NGS Prep System with Cancer All-In-One Assay on FFPE Samples
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"Comparison of Manual and Automated NGS Library Preparation Using the Magnis Dx NGS Prep System with Cancer All-In-One Assay on FFPE Samples"

The introduction of hybridization capture-based next-generation sequencing (NGS) methods into clinical practice helps laboratories obtain good quality data even from highly compromised samples such as DNA from formalin-fixed, paraffin-embedded (FFPE) or plasma samples. However, before these technologies can be implemented, molecular diagnostics laboratories need simplified, standardized, and reproducible library preparation protocols.

This white paper from Agilent shows how the Agilent SureSelect HS Cancer All-In-One Lung assay can be used to accurately confirm variants in non-small cell lung cancer FFPE samples with known mutations. The SureSelect assay also enables the detection of mutations in clinical cases where the genetic profile is unknown. Moreover, a direct comparison of manual library preparation with automated preparation using the Agilent Magnis DX NGS Prep system reveals that automation can provide greater yield and increased library complexity compared to manual methods.


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