HTG EdgeSeq is a targeted RNA sequencing platform based on quantitative nuclease protection chemistry, which requires substantially less sample input than other RNA-seq protocols. The automated library processor allows fast downstream analysis, making it a promising technology for clinical application and companion diagnostics, especially in samples from long-term storage or with a limited quantity of material.
This poster from HTG compares the use of HTG EdgeSeq and RNA-seq for detecting oncology biomarkers in a set of 57 formalin-fixed, paraffin-embedded tumor samples, finding that HTG EdgeSeq produced fewer QC failures while using less tissue input.
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