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Comparative Study of Via Extractor Tissue Disaggregator for Tissue Dissociation for Single-Cell RNA Sequencing Analysis
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"Comparative Study of Via Extractor Tissue Disaggregator for Tissue Dissociation for Single-Cell RNA Sequencing Analysis"

Single-cell sequencing is a powerful tool in the study of cellular heterogeneity among individual cells.

Advances in single-cell RNA sequencing (scRNA-seq), in particular, have enabled scientists to gain unprecedented insights into transcription profiles at the single-cell level, allowing the identification and study of rare cell populations in tissues of interest. Due to the high sensitivity of single-cell analyses, such as scRNA-seq, detailed attention must be put into experimental setup and execution. Careful handling and processing of human or animal tissue samples are critical to minimize any process-induced effects that may skew results.

This white paper from Cytiva investigates the ability of the Via Extractor tissue disaggregator to generate suspensions of high-quality single cells from fresh tissue for use in scRNA-seq analysis, finding it to provide a higher yield of viable mouse liver cells in a shorter period as compared to a competing dissociator.


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