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Centrifugation-Free Plasmid DNA Extraction: A Comparison of Magnetic Bead-Based Extraction Methods for Minipreps
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"Centrifugation-Free Plasmid DNA Extraction: A Comparison of Magnetic Bead-Based Extraction Methods for Minipreps"

Plasmid purification has recently moved into the spotlight with the development of mRNA vaccines for SARS-CoV-2. Aside from numerous virology applications, plasmid DNA (pDNA) is crucial in multiple cell and gene therapy (CGT) pipelines. Prior to large-scale production, obtaining high-quality pDNA in the preclinical development stages is necessary for the success of transformative medicine.

In preclinical vaccine and CGT development, the process of isolating pDNA from plasmids is limited by the requisite number of centrifugation steps needed during the plasmid extraction process. Centrifugation is a common technique used to separate pDNA from the host cell’s own genomic DNA. However, this technique is a rate-limiting step, especially in early-stage developmental workflows. Eliminating centrifugation may help relieve back-end bottlenecks and enable greater processing efficiencies, especially in high-throughput screening campaigns.

This white paper from Thermo Fisher Scientific compares the results of three magnetic bead-based extraction mini kits designed for pDNA isolation and purification. An analysis of manual and automated extraction techniques is discussed.


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