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Automated Affinity Purification of Immunoglobulins (IgGs) Using INtip Dispersive Solid-Phase Extraction Technology
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"Automated Affinity Purification of Immunoglobulins (IgGs) Using INtip Dispersive Solid-Phase Extraction Technology"

Solid-phase affinity extraction is a common laboratory method that is widely used in proteomics for protein purification from crude samples. Protein A immobilized resins designed to purify immunoglobulins (IgGs) are available in multiple formats, including magnetic beads, spin columns, and 96 well filter plates.

Resin-filled IMCStips offer an alternative approach, employing patented dispersive solid-phase extraction (dSPE) technology in a pipette tip (INtip purification) to allow multiple samples to be purified in parallel using multichannel pipettes. The protein purification protocol consists of multiple low speed pipetting cycles and washing steps. Automated liquid handling instruments are the ideal solution to relieve users of the strain caused by long manual pipetting sessions.

The affinity extraction protocol described in this application note demonstrates high recoveries, low background and reproducible antibody enrichment. IMCStips come with predefined pipetting protocols that are uploaded to the VIAFLO electronic pipette using INTEGRA’s VIALINK software. The ASSIST PLUS pipetting robot is then ready to perform the automated purification protocol, which takes around one hour. This combination of IMCS and INTEGRA Biosciences technologies offers scientists a fast, streamlined workflow that simplifies sample preparation and increases testing efficiency.


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