Genomic DNA anomalies are important biomarkers and drug targets in many cancer types.
DNA in-situ hybridization (ISH) is the standard method to directly visualize these molecular alterations, but currently available fluorescent ISH (FISH) assays provide limited morphological detail due to the use of fluorescent nuclear staining as compared to chromogenic staining. Furthermore, FISH techniques rely on fluorescence microscopes, risk the loss of fluorescent signal over time, and involve imaging at high magnifications. There is thus an unmet need for a sensitive and robust chromogenic DNA ISH assay that can enable high-resolution detection of genomic DNA targets with the ease of bright-field microscopy.
This white paper from ACD compares DNAscope — a novel chromogenic DNA ISH assay — to traditional ISH assays for cancer research applications, finding DNAscope to reliably detect various chromosomal structural aberrations at single-gene resolution with high sensitivity and specificity without requiring specialized lab equipment or personnel.
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