Precise tagging of genes via the homology-directed repair pathway is a versatile approach for studying gene function. Advances in genome editing technologies such as the CRISPR-Cas9 system have allowed researchers to target nearly any gene for modification. However, the efficiency of integrating large DNA molecules into the mammalian genome via HDR is inherently low.
This application note from Thermo Fisher Scientific describes a simple method for efficiently tagging endogenous genes using donor DNA prepared with the Invitrogen TrueTag Donor DNA Kit, GFP, with broad applications in general genome engineering, protein production, and immune cell therapy.
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