Upper and lower respiratory tract infections are caused by a broad range of microbes including RNA and DNA viruses, bacteria, and even fungi, and yet are often symptomatically similar. Detection of these pathogens can be challenging: immunoassays are limited to a small number of respiratory pathogens and lack sensitivity, whereas culture-based methods are labor intensive, have long turnaround times, and are prone to false-negative results due to fastidious growth in culture.
While molecular detection is more sensitive, most commercially available tests are expensive, primarily focus on either viruses or bacteria, and lack the flexibility to customize target lists based on laboratory needs. In addition, concurrent prevalence of viral and bacterial pathogens is a growing concern and needs effective detection methods.
This application note from Thermo Fisher Scientific presents data demonstrating the sensitivity and specificity of the Applied Biosystems TaqMan Array Respiratory Tract Microbiota Comprehensive Card in detecting a range of common and opportunistic respiratory pathogens and describes an end-to-end workflow for respiratory pathogen detection.
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