The microbiomics field has grown exponentially in recent years, but reproducibility and standardization of data remain a problem. Completely lysing of all types of microbes in a sample is challenging and can introduce extraction bias by underrepresenting species that are hard to lyse, such as Gram-positive bacteria.
To accommodate larger throughputs and reduce any bias associated with manual processing, microbiomics workflows require high throughput, unbiased, PCR inhibitor-free solutions for the extraction of sequencing-grade DNA from various sample types.
This application note from Tecan describes an automated workflow delivering pure, inhibitor-free, sequencing-grade DNA ready for downstream applications – including PCR, arrays, and sequencing – in 90 minutes. The note presents examples in which DNA was extracted from human fecal and skin swab samples and analyzed using 16S rRNA gene-targeted sequencing on an Illumina MiSeq System.
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