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"Next-Generation Sequencing Evaluation of QIAseq miRNA-Derived Libraries on the G4 Sequencing Platform"

MicroRNAs (miRNAs) are key players in nearly all normal and disease-related biological pathways and are a promising liquid biopsy-compatible biomarker type owing to their robust and stable expression in cell-free DNA and other biofluids.

Next-generation sequencing has emerged as a leading method for the analysis of miRNAs owing to its high sensitivity, precision, and ability to discriminate subtle but biologically relevant differences in miRNA abundance across healthy and disease samples. However, the short ~22 nucleotide length of miRNAs poses challenges for NGS library preparation and sequencing.

This application note from Singular Genomics demonstrates the use of the QIAseq miRNA Library Prep Kit in combination with the G4 Sequencing Platform for the analysis of human miRNA, showing the high sensitivity and accuracy and seamless integration of G4 sequencing data into the Qiagen GeneGlobe Data Analysis Portal.

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