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"Reference Materials for the Analysis of Methylation in Circulating Cell-Free DNA"

Methylation is an important epigenetic modification that influences cellular differentiation and gene expression.

Recently, liquid biopsies have started to incorporate analyses of epigenetic modifications for screening purposes to detect cancer-derived DNA in the blood. Additionally, epigenetic modifications are being used to also assign a tissue of origin to the cancer to direct confirmatory diagnostic procedures. However, the robust analysis of methylation is not trivial, nor is establishing the analytical validity of such assays.

This poster from LGC Clinical Diagnostics describes a set of differentially methylated reference materials for analyses of methylation in circulating cell-free DNA (ccfDNA) to enable the development, optimization, and validation of assays that assess the degree of methylation of ccfDNA across the genome and at specific sites.

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