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"Challenging the Limit of Detection in Liquid Biopsy Assays"

The low volume of plasma and few genome copies available in liquid biopsy assays limits the detection of variants at low allele frequencies, potentially causing false-negative results. Additionally, the fraction of cell-free DNA varies between cancer types, with circulating tumor DNA sometimes being as low as 0.1 percent of total DNA in a sample. Moreover, clonal hematopoiesis and errors introduced during library preparation can cause false-positive results.
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