The nanowell-based image-verified cloning method (HT-NIC: High-Throughput Nanowell-based Image-verified Single Cell Cloning), a new technique developed for the identification of monoclonal high-producer clones combines the CellCelector Flex with nanowell plates.
With this technique, it is possible to effectively assess and verify the clones before deciding which ones to choose. In less than one week, a pool of single cells develops into monoclonal, viable, and productive colonies. Instead of relying on large quantities of plates to produce a winner, users can now use actual data to reliably predict the future of clones.
This application guide from Sartorius presents a workflow for using the CellCelector nanowell-based image-verified cloning technology to screen and isolate cell clones for antibody discovery.
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