Lateral flow test strips faciltate user friendly, rapid, low-cost analysis for clinical diagnostics.
Advancements in lateral flow tests have largely focused on increasing the sensitivity of analyte detection.
The development of quantitative tests often uses organic fluorophores to achieve increased sensitivity. Pain points associated with fluorophores include ‘photobleaching’, which can compromise sensitivity. Europium chelate microspheres are an alternative to traditional fluorophores that can bypass this limitation.
This application note is a step-by-step guide to developing a quantitative lateral flow test using Estapor Europium Microspheres. The protocols for microsphere conjugation and test strip manufacture may be applied to different antibodies for the detection of alternative antigens.
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